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Protocol for correlation analysis of microbiome and metabolome using 16S rRNA sequencing and untargeted metabolomics

2023年5月14日 08:00
16S rRNA sequencing, bacterial diversity and community composition. unculturable and fastidious bacteria. interactions between the host and microbiota occur primarily through a multitude of metabolites. integrating analyses of the microbiome and metabolome should faciliate understanding the role of the microbiota in broiler … Spearman’s correlation analysis to identify microbe-metabolite relationships in different omics layers.

About

2024年9月19日 08:00
I was born in Tonghua, a small city which is located in Jilin Province, Northeastern China. I got my bachelor’s degree in Veterinary Medicine from China Agricultural University in 2018. I had a memorable time in CAU and also got familiar with Beijing. Later, I got my Ph.D. degree in Veterinary Microbiology from Iowa State University (ISU). ISU locates in Ames, a really beautiful and peaceful town in Iowa. I have many hobbies and I will not name them one by one here.

Spiral Plating and Automatic Colonies Counting

2023年2月19日 08:00
Eddy Jet 2W Spiral Plater EDDY JET 2W is a laboratory device used for spiral plating. It dispenses a precise amount of culture onto the agar plate in a controlled manner, producing a consistent and reproducible spiral pattern. This can save time and effort compared to manual plating techniques, and also reduces the risk of contamination. General description Components: a: main unit, b: disposable micro-syringe c: disposable sample beaker d: holder for micro-syringe e: holder for beakers box f: holder for 10 beakers Traditional serial dilution method Dilute Samples into suitable dilutions

师姐

2022年8月24日 08:00
如果说人生就像一列单程行驶的列车,那我真希望我是那个提前下车,挥手告别的人。 今天下午把师姐送到了得梅因机场附近的旅店,因为师姐明早要赶6点的

MALDI-tof

2022年8月8日 08:00
Step one Fill out the MALDI excel form, put the name of the isolates (2 spot per isolate), then two spots for BTS (control). Step two Apply samples on the plate, one sample has two consecutive spots.Also two spots BTS at the end. Add 1 uL 70% formic acid to each sample, but not BTS. Let the plate to be dry. Add 1 uL Matrix to each spot, including BTS.
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